Uključenost VEGF receptora i MAPK u kanabinoidnu potencijaciju Ca2+ fluksa u ćelije neuroblastoma N18TG2

From Kanabstrakt
Jump to: navigation, search
Uključenost VEGF receptora i MAPK u kanabinoidnu potencijaciju Ca2+ fluksa u ćelije neuroblastoma N18TG2
Časopis

Molecular Brain Research

Godina 2004
Autori

Rubovitch V , Gafni M , Sarne Y.

Institucija The Mauerberger Chair in Neuropharmacology, Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, 69978, Israel
PubMed

PMID:14741403

Free text URL
Tip članka Laboratorijsko istraživanje In Vitro



Naslov originala

The involvement of VEGF receptors and MAPK in the cannabinoid potentiation of Ca2+ flux into N18TG2 neuroblastoma cells

Originalni apstrakt

In addition to their inhibitory effects, cannabinoids also exert stimulatory activity which can be detected at the cellular level. In a previous study, we demonstrated a stimulatory effect of the synthetic cannabinoid receptor agonist desacetyllevonantradol (DALN) on Ca(2+) flux into N18TG2 neuroblastoma cells, and suggested a dual mechanism: one pathway mediated by PKA and the other one by protein kinase C (PKC). Here we studied the PKC-mediated effect of DALN on Ca(2+) influx. The stimulatory effect of DALN on Ca(2+) influx was partially blocked by the PKC inhibitor chelerythrine, by the metalloprotease inhibitor o-phenanthroline and by the MEK (mitogen-activated protein-kinase kinase, MAPK kinase) inhibitor PD98059. Immunobloting of ERK1/2 MAPK demonstrated phosphorylation by DALN, and indicated the involvement of vascular endothelial growth factor (VEGF) receptor tyrosin kinases (RTKs) in MAPK activation as it was blocked by oxindole-1. Transactivation of the VEGFR-MAPK cascade by DALN involved CB1 cannabinoid receptors coupled to Gi/Go GTP-binding proteins as it was blocked by SR141716A and by pertussis toxin (PTX). The pharmacological implications of this novel mechanism of cannabinoid activity are discussed.

COPYRIGHT: © Elsevier Inc. All rights reserved.


Prevod naslova

Uključenost VEGF receptora i MAPK u kanabinoidnu potencijaciju Ca2+ fluksa u ćelije neuroblastoma N18TG2

Prevod apstrakta

Ovaj članak nije preveden! Pomozite Kanabis Apstraktima tako što ćete dodati prevod na srpsko-hrvatski.






"<span class="error">Error: no local variable "c" was set.</span>" is not a number.